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Histone H3 Recombinant Rabbit mAb (HRP Conjugate) (SDT-266-44)

Histone H3 Recombinant Rabbit mAb (HRP Conjugate) (SDT-266-44)

货号: S0B1622
价格: 720
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3

  • 分子别名

    Histone H3.1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    SDT-266-44

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, mouse brain, C6

  • 预测反应种属
    (反应种属缩写表)

    Ar, Pl, SeUr, Dr, Hm, C.el, Or, Bv, Xe

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:5000-1:10000 Hu, Ms, Rt
背景介绍

  • Histone H3 is a critical component of the nucleosome, which is the basic unit of chromatin. It plays a vital role in the regulation of gene transcription and is subject to various post-translational modifications (PTMs) that affect chromatin structure and gene expression. The H3 family includes several variants, with H3.1, H3.2, and H3.3 being the most well-known. H3.1 and H3.2 are deposited during DNA replication, while H3.3 can be deposited independently of DNA replication and is incorporated throughout the cell cycle2. Post-translational modifications of Histone H3, particularly on the N- and C-terminal tails, can influence the interaction between histones and DNA, as well as with other chromatin factors. These modifications can either promote a relaxed chromatin state that allows gene transcription or lead to a condensed chromatin state that typically suppresses transcription.

  • 免疫印迹

    • WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: HeLa whole cell lysate 20 µg
      Primary antibody:
      Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa

    • WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: mouse brain lysate 20 µg
      Primary antibody:
      Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa

    • WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: C6 whole cell lysate 20 µg
      Primary antibody:
      Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa