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α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) (SDT-311-20)

α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) (SDT-311-20)

货号: S0B1621
价格: 720
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    α-Actinin-1

  • 分子别名

    Alpha-actinin-1; ACTN1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoskeleton, Cell membrane, Cytoplasm

  • Accession

    P12814

  • 克隆号

    SDT-311-20

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:5000-1:10000 Hu, Ms, Rt
背景介绍

  • Alpha-actinin-1 is a member of the spectrin superfamily of proteins and is an actin-binding protein with multiple roles in different cell types1. It is a component of the cytoskeleton and is involved in cross-linking actin filaments, which helps in maintaining cell shape, providing mechanical strength, and playing a role in cell motility and signal transduction. In nonmuscle cells, alpha-actinin-1 is found along microfilament bundles and adherens-type junctions, where it binds actin to the membrane. It is also present in muscle cells, where it helps anchor the myofibrillar actin filaments at the Z-discs and dense bodies. This protein is highly conserved and has been implicated in various biological processes, including cell migration, cell division, and the maintenance of cell structure. It is expressed ubiquitously and has been associated with several diseases. For instance, alpha-actinin-1 has been linked to myopathies, neurodegenerative disorders, and kidney diseases. It may also play a role in cancer cell progression and metastasis, as well as in the migration of immune cells. In terms of autoimmune diseases, alpha-actinin-1 has been identified as a potential target autoantigen, particularly in systemic lupus erythematosus (SLE) and autoimmune hepatitis. It is suggested that antibodies against alpha-actinin-1 may contribute to the pathogenesis of these diseases by cross-reacting with other proteins.

  • 免疫印迹

    • WB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: HeLa whole cell lysate 20 µg; HepG2 whole cell lysate 20 µg
      Primary antibody:
      Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 103 kDa
      Observed MW: 103 kDa

    • WB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: NIH/3T3 whole cell lysate 20 µg
      Primary antibody:
      Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 103 kDa
      Observed MW: 103 kDa

    • WB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: C6 whole cell lysate 20 µg
      Primary antibody:
      Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)) at 1/5000 dilution
      Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 103 kDa
      Observed MW: 103 kDa