您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
Rat mAb IgG2b Isotype Control (S-R486)

Rat mAb IgG2b Isotype Control (S-R486)

货号: S0B1028
价格: 500
规格: 0.1mg
介绍: -
其他: -
产品规格

  • 宿主来源

    Rat

  • 抗原名称

    Rat IgG2b

  • 克隆号

    S-R486

  • 抗体类型

    Rat mAb

  • 抗体同种型

    IgG2b,k

  • 应用

    IHC-P, FCM

  • 纯化方式

    Protein G

  • 浓度

    0.2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
背景介绍

  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Rat mAb IgG2b Isotype Control at 1/20 dilution (1 μg) / (Left panel) compared with CD8α antibody at 1/200 (1 μg) dilution (S0B0498) / (Right panel). Goat Anti-Rat IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ separately. Gated on total viable cells.

  • 免疫组化

    • IHC shows negative staining in paraffin-embedded mouse spleen. Rat mAb IgG2b Isotype Control was used at 1/80 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.