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Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (S-933-228)

Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (S-933-228)

货号: S0B1618
价格: 650
规格: 20T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    Integrin beta1/CD29
  • 分子别名

    Fibronectin receptor subunit beta; Glycoprotein IIa (GPIIA); VLA-4 subunit beta; ITGB1; FNRB; MDF2; MSK12
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane
  • Accession

    P05556
  • 克隆号

    S-933-228
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 阳性样本

    human PBMC
  • 纯化方式

    Protein G
  • 浓度

    1mg/ml
  • 标记

    Alexa Fluor® 488
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度 推荐种属
FCM 5 μl per million cells in 100μl volume Hu
背景介绍
  • CD29 is an integrin component that mediates adhesion and involves in homing to sites of inflammation. It expresses in fibroblasts, platelets, T cells, monocytes, granulocytes(low), mast cells, endothelial cells and myoepithelium, also other diverse cell types. It does not express in red blood cells and spermatogonia. It is a myoepithelial marker, although established markers (SMA, CD10, p63, S100, maspin, calponin, GFAP, smooth muscle myosin) are more commonly used.

  • 流式分析

    • Flow cytometric analysis of Human Integrin beta1/CD29 expression on human PBMC (human peripheral blood mononuclear cell). Cells Fluorescent histograms were gated events with monocytes (Right) or lymphocytes (Left) was stained with either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (Red line histogram) at 5 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
  • 实验方案