您现在的位置: 首页   -  产品中心   -  免疫学   -   抗体
CD3 epsilon Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-241-49)

CD3 epsilon Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-241-49)

货号: S0B1610
价格: 458
规格: 10μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    CD3 EPSILON
  • 分子别名

    T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; T3E; CD3E
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cell membrane
  • Accession

    P07766
  • 克隆号

    SDT-241-49
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICC, IF
  • 反应种属 ?

    Hu
  • 阳性样本

    Human tonsil
  • 纯化方式

    Protein A
  • 浓度

    2 mg/ml
  • 标记

    Alexa Fluor® 488
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
ICC 1:200 Hu
IF 1:50-1:100 Hu
背景介绍
  • CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.

  • 免疫细胞化学

    • ICC shows positive staining in Jurkat cells. Anti- CD3 epsilon (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows negative staining in Ramos cells. Anti- CD3 epsilon (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded Human tonsil. Anti-CD3 epsilon (Alexa Fluor® 488 Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.