12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:200 |
Cyclophilin B, also known as CypB or PPIB, is an enzyme that belongs to the family of peptidyl-prolyl isomerases (PPIases). It plays a vital role in protein folding, a process that is essential for the proper functioning of proteins within the cell. Cyclophilin B is involved in various cellular processes, including the immune response, and has been implicated in the pathogenesis of several diseases, such as cancer and viral infections. In the context of viral infections, Cyclophilin B has been shown to interact with the hepatitis C virus (HCV) NS5B polymerase, enhancing its RNA-binding activity, which is crucial for viral replication. Inhibitors of cyclophilin B have demonstrated antiviral activity against HCV, both in vitro and in vivo, suggesting a potential therapeutic target for the treatment of HCV infection. Moreover, Cyclophilin B has been reported to be overexpressed in various cancers, including non-small cell lung carcinoma, hepatocellular carcinoma, and pancreatic carcinoma. Its overexpression has been correlated with reduced patient survival, indicating a possible role in tumor progression.
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Weak expression: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: dog liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: chicken liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
IHC shows positive staining in paraffin-embedded human liver. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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