PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
| FCM | 1:500 |
Isotype control antibodies, to estimate the nonspecific binding of target.
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling rat IgG2a isotype control antibody at 1/500 dilution (1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
IHC shows negative staining in paraffin-embedded mouse kidney. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse spleen. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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