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β-tubulin Recombinant Rabbit mAb (HRP Conjugate) (S-R014)

β-tubulin Recombinant Rabbit mAb (HRP Conjugate) (S-R014)

货号: S0B1593
价格: 480
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    β-tubulin

  • 分子别名

    Tubulin beta chain, Tubulin beta-5 chain, TUBB5, TUBB

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P07437

  • 克隆号

    S-R014

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:5000-1:10000
背景介绍

  • Beta-tubulin is a member of the tubulin protein family, which forms the structural basis of microtubules, essential components of the cytoskeleton in eukaryotic cells. These microtubules play a crucial role in various cellular processes, including mitosis, intracellular transport, and cell signaling. Beta-tubulin associates with alpha-tubulin to create heterodimers, which then polymerize into microtubules. It is particularly important during the formation of the mitotic spindle, facilitating chromosome segregation. Beta-tubulin is also involved in the stabilization of microtubules and can be a target for antimitotic drugs, making it significant in cancer therapy. Its expression and activity can vary among different cell types and developmental stages, highlighting its versatility in cellular functions.

  • 免疫印迹

    • WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: HeLa whole cell lysate 20 µg
      Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: NIH/3T3 whole cell lysate 20 µg; mouse brain lysate 20 µg
      Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: rat brain lysate 20 µg
      Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa