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PSEN1 Recombinant Rabbit mAb (S-1002-129)

PSEN1 Recombinant Rabbit mAb (S-1002-129)

货号: S0B0905
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    PSEN1
  • 分子别名

    Presenilin-1, PS-1, Protein S182, AD3, PS1, PSNL1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Golgi apparatus membrane, Cell membrane, Endoplasmic reticulum membrane
  • Accession

    P49768
  • 克隆号

    S-1002-129
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICFCM, IHC-P, ICC, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 预测反应种属
    (反应种属缩写表)

    Mq, Or
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:100
ICC 1:500
ICFCM 1:50
背景介绍
  • PSEN1 is a protein involved in the gamma-secretase complex, which is crucial for the processing of several proteins, including the amyloid precursor protein (APP) and the Notch receptor. Mutations in the PSEN1 gene are associated with early-onset familial Alzheimer's disease (AD) and can lead to an increased production of amyloid-beta, the main component of amyloid plaques found in AD brains. Over 300 PSEN1 mutations have been discovered, some of which have been found in atypical AD or non-AD phenotypes such as frontotemporal dementia (FTD), Parkinson's disease (PD), dementia with Lewy bodies (DLB), or spastic paraparesis (SP).

  • 免疫印迹

    • WB result of PSEN1 Recombinant Rabbit mAb
      Primary antibody: PSEN1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 53 kDa
      Observed MW: 18 kDa

    • WB result of PSEN1 Recombinant Rabbit mAb
      Primary antibody: PSEN1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 53 kDa
      Observed MW: 18 kDa

    • WB result of PSEN1 Recombinant Rabbit mAb
      Primary antibody: PSEN1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 53 kDa
      Observed MW: 18 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling PSEN1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PSEN1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-PSEN1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PSEN1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PSEN1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PSEN1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in MCF7 cells. Anti- PSEN1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).