PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
mIHC | 1:100-1:2000 |
PD-L1, Regulatory T cell, Cytotoxic T cell and Macrophage Marker (PD-L1, FOXP3, CD8α, CD68, CD163) Antibody Panel contains 5 recombinant monoclonal antibodies against PD-L1, FOXP3, CD8α, CD68 and CD163. They are provided as a sampler panel. Panel contains: - PD-L1 Recombinant Rabbit mAb (SDT-R017) S0B2134 - S-RMab® FOXP3 Recombinant Mouse mAb (SDT-R149) S0B2193 - CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148 - S-RMab® CD68 Recombinant Rabbit mAb (SDT-R025) S0B2064 - S-RMab® CD163 Recombinant Rabbit mAb (SDT-R164) S0B2194
mIHC shows positive staining in paraffin-embedded human tonsil (A). Anti-CD8α Mouse mAb (S0B2148) was used at 1/500 dilution (Red, B), Anti- PD-L1 Rabbit mAb (S0B2134) was used at 1/200 dilution (Yellow, C), Anti-CD68 Rabbit mAb (S0B2064) was used at 1/500 dilution (Orange, D), Anti-CD163 Rabbit mAb (S0B2194) was used at 1/2000 dilution (magenta, E), and Anti-FOXP3 Mouse mAb (S0B2193) was used at 1/100 dilution (Green, F), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.