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CD45 Recombinant Rat mAb (S-R477)

CD45 Recombinant Rat mAb (S-R477)

货号: S0B0909
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rat
  • 抗原名称

    CD45
  • 分子别名

    Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC, Lymphocyte antigen 5 (Ly-5)
  • 细胞定位

    Cell membrane
  • Accession

    P06800
  • 克隆号

    S-R477
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG2b,k
  • 应用

    FCM
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein G
  • 浓度

    2 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
FCM 1:2000
背景介绍
  • CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.

  • 流式分析

    • Flow cytometric analysis of mouse primary splenocytes labelling CD45 antibody at 1/2000 dilution (0.1 μg)/ (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Gated on total viable cells.