PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling FITC Mouse IgA, κ Isotype Control Antibody at 1/20 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG FITC was used as the secondary antibody) (Left) / (Black) compared with FITC Mouse IgA, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling FITC Mouse IgA, κ Isotype Control Antibody at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG FITC was used as the secondary antibody) (Left) / (Black) compared with FITC Mouse IgA, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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