PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| IHC-P | 1:200-1:1000 |
| ICC | 1:500 |
| ICFCM | 1:500 |
p38 MAPK, a member of the mitogen-activated protein kinase (MAPK) family, is activated by a variety of environmental stresses and inflammatory cytokines. p38 MAPK family includes p38α, p38β, p38γ, and p38δ. This pathway plays a crucial role in the regulation of cellular responses to stress, inflammation, and immune function. The p38 MAPK pathway is initiated by MAPK kinase kinases (MAPKKKs), such as MEKK or MLK, which phosphorylate and activate MAPK kinases (MAPKKs) like MKK3/6. These, in turn, phosphorylate and activate p38 MAPK. Downstream of p38 MAPK, a number of substrates are regulated, including HSP27, MAPKAPK-2 (MK2), MAPKAPK-3 (MK3), and various transcription factors like ATF-2, Stat1, Max/Myc complex, MEF-2, and Elk-1. This signaling pathway is involved in a wide range of biological processes, from the regulation of gene expression to cell cycle control, apoptosis, and autophagy. The p38 MAPK pathway is also implicated in the pathogenesis of various diseases, including cancer, where it can act as either a promoter or suppressor of tumor growth and metastasis, depending on the cellular context.
WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling p38α MAPK+p38β MAPK antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling p38α MAPK+p38β MAPK antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
p38α MAPK+p38β MAPK Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p38α MAPK+p38β MAPK in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using p38α MAPK+p38β MAPK Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: p38α MAPK+p38β MAPK Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 41 kDa
Observed MW: 38 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-p38α MAPK+p38β antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p38α MAPK+p38β antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti-p38α MAPK+p38β MAPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-p38α MAPK+p38β MAPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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