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Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb (S-617-138)

Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb (S-617-138)

货号: S0B0897
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-p38 MAPK (Tyr182)

  • 分子别名

    Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein; CSBP), MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha), Stress-activated protein kinase 2a (SAPK2a), CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q16539

  • 克隆号

    S-617-138

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB, IP

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Zf, Fs, Cz, Dg, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
Dot Blot 1:1000
WB 1:1000
IP 1:50
ICC 1:500
背景介绍

  • p38 mitogen-activated protein kinases are a class of mitogen-activated protein kinases (MAPKs) that are responsive to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock, and are involved in cell differentiation, apoptosis and autophagy. Abnormal activity (higher or lower than physiological) of p38 has been implicated in pathological stresses in several tissues, that include neuronal, bone, lung, cardiac and skeletal muscle, red blood cells, and fetal tissues. Phospho-p38 MAPK (Tyr182), often tested alongside Thr180, is a significant biomarker for the activation state of p38 MAPK, a crucial enzyme in cellular stress and inflammatory responses. The phosphorylation at these sites, Thr180 and Tyr182, is essential for the full activation of p38 MAPK, allowing it to regulate various cellular processes in response to stress, inflammation, and other signals.

  • 免疫印迹

    • WB result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
      Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated Jurkat whole cell lysate 20 µg
      Lane 2: Jurkat treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 41 kDa
      Observed MW: 38 kDa

    • WB result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
      Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 41 kDa
      Observed MW: 38 kDa

  • 斑点杂交

    • Dot blot result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
      Lane 1: Phospho-p38 MAPK (Thr180/Tyr182) peptide
      Lane 2: p38 MAPK WT peptide
      Lane 3: Phospho-p38 MAPK (Thr180) peptide
      Lane 4: Phospho-p38 MAPK (Tyr182) peptide
      Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫细胞化学

    • ICC analysis of Jurkat cells treated with Anisomycin (25μM, 30min) (top panel) and Jurkat cells untreated with Anisomycin (25μM, 30min) (below panel). Anti-Phospho-p38 MAPK (Tyr182) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).