sGCα1：95KD, sGCβ1:70KD
>85% by SDS-PAGE
-80℃
The sGCα1/sGCβ1 activity was detected using TR-FRET technology. The reaction was performed by incubating the sGCα1/sGCβ1 protein and varying concentration of DETA NONOate at 25℃ for 10 min, adding the GTP and the detection reagent at 25℃ for 60 min, then reading signal ratio with BMG.
M MW Standard
1 1 μg sGCα1/sGCβ1
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