Gln32-Ser728
80 kDa (Non-Reducing)
＞95% by SDS-PAGE
PBS, PH7.4
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1、Review Front Pharmacol . 2022 Mar 17:13:808855. eCollection 2022.
HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases and contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The EC50 for this effect is less than 1ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
92.789%
Immobilized HGF Protein, Human (Cat. No. UA040194) at 2.0μg/mL (100μL/well) can bind HGFR/c-MET Fc Chimera Protein, Human (Cat. No. UA010829) with EC50 of 23.39-29.12 ng/mL.
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