USP15 Protein

The fundamental role of USP15 is specific removal of ubiquitin from substrates. USP15 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader

Lane M: Marker

Lane 1: 2 μg, reduced

Lane 2: 2 μg, non-reduced