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Phospho-CaMKII (Thr286) Recombinant Rabbit mAb (S-1251-278)

Phospho-CaMKII (Thr286) Recombinant Rabbit mAb (S-1251-278)

货号: S0B6070
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-CaMKII (Thr286)

  • 分子别名

    Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMKA; KIAA0968; CAMK2A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Synapse

  • Accession

    Q9UQM7

  • 克隆号

    S-1251-278

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Human brain, mouse brain, rat brain

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Ms, Rt
背景介绍

  • Phospho-CaMKII (Thr286) refers to the phosphorylated form of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) at threonine residue 286. CaMKII is a multifunctional serine/threonine protein kinase that plays a crucial role in various cellular processes, particularly in the heart and brain. When phosphorylated at Thr286, CaMKII undergoes autophosphorylation, which enhances its activity and allows it to function in a Ca2+-independent manner. This phosphorylated state is essential for the regulation of cardiac myocyte contraction, synaptic plasticity, and memory formation. Abnormal phosphorylation of CaMKII at Thr286 has been implicated in several pathological conditions, including heart failure and arrhythmias, making it a potential therapeutic target for cardiovascular diseases.

  • 免疫印迹

    • WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated mouse brain lysate 20 µg
      Lane 2: Lambda phosphatase treated mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 48, 60 kDa

    • WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated rat brain lysate 20 µg
      Lane 2: Lambda phosphatase treated rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 48, 60 kDa

  • 斑点杂交

    • Dot blot result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
      Lane 1: CaMKII (Thr286) phospho peptide
      Lane 2: CaMKII unmodified peptide
      Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution