PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Phospho-Rb (Ser807/811) protein refers to the phosphorylated form of the retinoblastoma protein (Rb) at serine residues 807 and 811. The retinoblastoma protein is a crucial tumor suppressor that plays a pivotal role in regulating cell cycle progression, particularly in controlling the transition from the G1 to S phase. When phosphorylated at Ser807/811, Rb undergoes a conformational change that allows it to release the transcription factor E2F, which in turn promotes cell cycle progression. This phosphorylation is mainly mediated by cyclin-dependent kinases (CDKs) such as CDK4 and CDK6, and it is tightly regulated to ensure proper cell cycle control. Abnormal phosphorylation of Rb at these sites is often associated with various cancers, highlighting its significance in maintaining genomic stability and preventing uncontrolled cell proliferation.
WB result of Phospho-Rb (Ser807/811) Recombinant Rabbit mAb
Primary antibody: Phospho-Rb (Ser807/811) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated WI-38 whole cell lysate 20 µg
Lane 2: WI-38 starve for 3 days, then treated with 10 % serum for 2 days whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 115 kDa
WB result of Phospho-Rb (Ser807/811) Recombinant Rabbit mAb
Primary antibody: Phospho-Rb (Ser807/811) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C2C12 whole cell lysate 20 µg
Lane 2: Lambda phosphatase treated C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 115 kDa
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer (Left) and negative staining in human colon cancer treated with phosphatase (Right). Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC analysis of MCF7 cells treated with Lambda PP for 1 hr (top panel) and MCF7 cells untreated with Lambda PP (below panel). Anti- Phospho-Rb (Ser807/811) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of Phospho-Rb (Ser807/811) in tumor tissue.
Expression of Phospho-Rb (Ser807/811) in human tissue.
Expression of Phospho-Rb (Ser807/811) in mouse & rat tissue.
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