PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
C57BL/6 mouse splenocytes were stained with SDT Alexa Fluor® 488 rat IgG2b isotype control, anti-keyhole limpet hemocyanin at 1/20 dilution (1 μg) (Red line histogram). Cells without incubation with primary antibody and secondary antibody (Black line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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