PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 1.25μl per million cells in 100μl volume | Hu |
CD294, also known as CRTH2, PTGDR2, or GPR44, is a seven-transmembrane G protein-coupled receptor primarily expressed on Th2 cells, eosinophils, basophils, and type-2 innate lymphoid cells (ILC2). It is involved in mediating the chemotaxis of these cells to sites of allergic inflammation, such as the asthmatic lung, and plays a role in preventing apoptosis in Th2 cells.
Flow cytometric analysis of Human CD294 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with FITC Mouse Anti-Human CD123 antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rat Anti-Human CD294 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.