12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Rat IL-2 (Interleukin-2) is a 15–17 kDa glycoprotein and key T-cell growth factor primarily secreted by activated CD4+ T cells, NK cells, and dendritic cells. It drives T-cell proliferation, Th1 differentiation, and NK cell activity while also supporting regulatory T cell (Treg) function for immune tolerance. Signaling through the IL-2 receptor (IL-2Rαβγ) activates JAK-STAT pathways, promoting cell survival and expansion. Rat IL-2 shares ~70% homology with mouse IL-2 but differs significantly from human IL-2, requiring species-specific detection methods (e.g., ELISA, flow cytometry). It is crucial in research on autoimmunity (e.g., EAE), transplantation, and cancer immunotherapy, with dysregulation linked to immune disorders. Recombinant IL-2 has therapeutic potential but demands rat-specific reagents for accurate study.
Standard curve
Example of Rat IL-2 standard curve in Assay Diluent #12.
Linearity
The concentrations of Rat IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Rat spleen stimulated with 5ug/ml Concanavalin A for 2days (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 40.49 ng/mL in stimulated Rat spleen supernatant.
Linearity
The sample is undiluted samples are as follows: Rat spleen for 2days (12.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.09 ng/mL in unstimulated Rat spleen supernatant.
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