PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Cleaved Caspase-8 (Asp374) is a key protein involved in the extrinsic apoptosis pathway, which is activated by death receptors such as Fas (CD95) and tumor necrosis factor receptor 1 (TNFR1). When cleaved at Asp374, it generates active fragments, including the p18 subunit, which can further activate downstream effector caspases like caspase-3, -6, and -7, ultimately leading to apoptosis. This protein is also implicated in regulating inflammatory necrotic cell death pathways such as necroptosis and pyroptosis.
WB result of Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 10 μg/ml Cycloheximide for 18 hours, then treated with 20 ng/ml hTNF-α for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 43, 60 kDa
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