PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Rt |
Dihydroorotate dehydrogenase (DHODH) is a crucial enzyme located in the inner mitochondrial membrane, playing a vital role in the de novo pyrimidine biosynthesis pathway. It catalyzes the conversion of dihydroorotate to orotate, which is the fourth and only redox step in this pathway. This process is essential for the production of pyrimidine nucleotides, which are fundamental for DNA and RNA synthesis. DHODH is a flavoprotein that relies on ubiquinone (CoQ) for its function, and its activity is closely linked to the mitochondrial electron transport chain. Additionally, DHODH has garnered attention in cancer research due to its role in tumor cell proliferation and its potential as a target for cancer treatment.
WB result of DHODH Recombinant Rabbit mAb
Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 50 kDa
WB result of DHODH Recombinant Rabbit mAb
Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
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