PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms |
PIK3CD is a gene that encodes a protein which is an integral part of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. This pathway is crucial for various cellular functions including cell growth, proliferation, survival, and metabolism. The PIK3CD protein specifically refers to the delta isoform of the catalytic subunit of PI3K. It is predominantly expressed in immune cells and plays a significant role in the regulation of immune responses. Abnormal activation or mutations in the PIK3CD gene can lead to a range of diseases including certain types of leukemia and other immune-related disorders. Understanding its function and regulation is important for developing targeted therapies in cancer and immune system disorders.
WB result of PIK3CD Recombinant Rabbit mAb
Primary antibody: PIK3CD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
WB result of PIK3CD Recombinant Rabbit mAb
Primary antibody: PIK3CD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
IHC shows positive staining in paraffin-embedded human colon. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of PIK3CD in tumor tissue.
Expression of PIK3CD in human tissue.
Expression of PIK3CD in mouse & rat tissue.
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