12 months from date of receipt / reconstitution, 2 to 8'C as supplied.
The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
Standard curve
Example of Human IFN-γ standard curve in Assay Diluent #11E5.
Linearity
The concentrations of Human IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6days (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 55 ng/mL in stimulated human PBMC supernatant.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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