PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:200 | Hu |
| IF | 1:200 | Hu |
| ICFCM | 1:500 |
Stat6 protein, which stands for Signal Transducer and Activator of Transcription 6, is a crucial component in the immune system. It is a member of the Stat family of transcription factors that play key roles in transmitting signals from cytokine receptors to the nucleus. When activated by cytokines like IL-4 and IL-13, Stat6 translocates to the nucleus and regulates the expression of numerous genes involved in immune responses, such as those related to the differentiation of T helper 2 cells and the production of immunoglobulin E. This protein is essential for mounting effective immune responses against certain pathogens and also has implications in various immune-related diseases, including asthma and allergic reactions.
ICC shows positive staining in HeLa cells. Anti- Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (meganta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded human colon. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human kidney. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
您现在的位置：