PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICFCM | 5μl per million cells in 100μl volume | Rt |
The CD68 protein is a heavily glycosylated transmembrane protein belonging to the lysosome-associated membrane glycoprotein (LAMP) family, primarily serving as a macrophage marker. With a molecular weight of approximately 110 kDa, it contains scavenger receptor-like cysteine-rich domains and participates in innate immune responses by binding specific ligands such as oxidized low-density lipoprotein (oxLDL). Localized to lysosomal and endosomal membranes, CD68 plays a crucial role in antigen presentation, inflammatory responses, and tissue repair. It is commonly detected via immunohistochemistry (e.g., using the PG-M1 antibody) and is widely utilized to identify tumor-associated macrophages (TAMs) and assess pathological progression in chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis.
Lewis Rat peritoneal exudates cells were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Phycoerythrin Mouse Anti-Rat CD11b and SDT FITC Mouse Anti-Rat CD68 Antibody Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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