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APC Rabbit Anti-Human Glypican-3 Antibody (SDT-R032)

APC Rabbit Anti-Human Glypican-3 Antibody (SDT-R032)

货号: S0B5497
价格: 650
规格: 25T
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Glypican-3
  • 分子别名

    GTR2-2; Intestinal protein OCI-5; MXR7; OCI5; GPC3
  • 细胞定位

    Cell membrane
  • Accession

    P51654
  • 克隆号

    SDT-R032
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 阳性样本

    HepG2
  • 纯化方式

    Protein A
  • 浓度

    50 μg/ml
  • 标记

    APC
  • 性状

    Liquid
  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度 推荐种属
FCM 5μl per million cells in 100μl volume Hu
背景介绍
  • Glypican-3 (GPC3) is a cell-surface glycoprotein that is highly expressed in hepatocellular carcinoma (HCC) and some other cancers. It is a member of the glypican family of heparan sulfate proteoglycans, consisting of a core protein and heparan sulfate chains, and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. GPC3 is encoded by the GPC3 gene located on the X chromosome and is cleaved by furin into an N-terminal 40 kDa subunit and a C-terminal 30 kDa subunit. It plays a role in cell proliferation, morphogenesis, and oncogenesis by interacting with signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor. Abnormal expression of GPC3 is associated with poor prognosis in cancer, making it a potential target for cancer diagnosis and immunotherapy.

  • 流式分析

    • Flow cytometric analysis of Human Glypican-3 expression on HepG2 cells. Cells from the HepG2 (Human hepatocellular carcinoma epithelial cell, Right) or A549 (Human lung carcinoma epithelial cell, Left) was stained with either APC Rabbit IgG Isotype Control (Black line histogram) or SDT APC Rabbit Anti-Human Glypican-3 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.