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Actin (plant specific) Rabbit Polyclonal Antibody

Actin (plant specific) Rabbit Polyclonal Antibody

货号: S0B6046
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Actin (plant specific)
  • 分子别名

    Actin-2; ACT2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Cytoskeleton
  • Accession

    Q96292
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 应用

    WB
  • 反应种属 ?

    Arabidopsis, Rice, Soybean
  • 阳性样本

    Arabidopsis, rice, soybean
  • 纯化方式

    Immunogen Affinity
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Arabidopsis, Rice, Soybean
背景介绍
  • Actin in plants is a highly conserved cytoskeletal protein that plays essential roles in maintaining cell shape, cytoplasmic streaming, cell division, cell expansion, and intracellular transport. Similar to animal cells, plant actin exists in two forms: monomeric G-actin (globular) and polymeric F-actin (filamentous), which dynamically assemble into microfilament networks to regulate cellular processes. Plants possess multiple actin isoforms (e.g., ACT2, ACT7), each exhibiting tissue-specific and developmental stage-dependent expression patterns—for instance, ACT7 is highly expressed in rapidly growing tissues. Actin filaments interact with various binding proteins, including myosin, formin, and profilin, to drive organelle movement (e.g., chloroplast positioning), cytoplasmic streaming, and critical developmental processes such as cell plate formation, pollen tube tip growth, and stomatal movement. Additionally, plant actin undergoes rapid reorganization in response to environmental stimuli (e.g., gravity, mechanical stress, and pathogen attack), enabling adaptation to external challenges. While the actin gene family is evolutionarily conserved, plants have undergone unique gene duplication and functional diversification to support their distinct growth and developmental strategies.

  • 免疫印迹

    • WB result of Actin (plant specific) Rabbit pAb
      Primary antibody: Actin (plant specific) Rabbit pAb at 1/1000 dilution
      Lane 1: arabidopsis whole plant lysate 20 µg
      Lane 2: rice whole plant lysate 20 µg
      Lane 3: soybean whole plant lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa