PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
CD99, also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein encoded by the CD99 gene. It is widely expressed in various cell types, including hematopoietic cells, endothelial cells, and many types of cancer cells. This protein plays crucial roles in multiple biological processes such as cell adhesion, migration, differentiation, and apoptosis. In the immune system, CD99 is involved in T-cell activation, maturation, and the regulation of leukocyte transmigration. It also has a significant impact on tumor biology, as it is often overexpressed in cancers like Ewing sarcoma and certain leukemias. Engagement of CD99 by specific antibodies can induce caspase-independent cell death in tumor cells, making it a potential therapeutic target.
WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: U-937 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Lane 4: HuT-78 whole cell lysate 20 µg
Lane 5: HUVEC whole cell lysate 20 µg
Lane 6: THP-1 whole cell lysate 20 µg
Lane 7: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
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