PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
CMTM6, also known as CKLF-like MARVEL transmembrane domain-containing 6, is a transmembrane protein encoded by the CMTM6 gene, located on chromosome 3. It belongs to the CKLF-like MARVEL transmembrane domain-containing family, which consists of nine members and is involved in various biological processes including autoimmune diseases, cardiovascular diseases, the male reproductive system, hematopoiesis, and cancer development. CMTM6 has been identified as a critical regulator of PD-L1, an immune checkpoint protein. It stabilizes PD-L1 on the cell surface by preventing its lysosome-mediated degradation and ubiquitination, thereby enhancing the ability of tumor cells to evade immune surveillance. High expression of CMTM6 is associated with poor prognosis in several types of cancer, such as glioma, hepatocellular carcinoma, and triple-negative breast cancer.
WB result of CMTM6 Recombinant Rabbit mAb
Primary antibody: CMTM6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled MDA-MB-231 whole cell lysate 20 µg
Lane 2: unboiled Raji whole cell lysate 20 µg
Lane 3: unboiled BxPC-3 whole cell lysate 20 µg
Lane 4: unboiled HCT 116 whole cell lysate 20 µg
Lane 5: unboiled HDLM-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 17-20 kDa
IHC shows positive staining in paraffin-embedded human spleen. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Low expression: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CMTM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of CMTM6 in tumor tissue.
Expression of CMTM6 in human tissue.
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