Interferon gamma (IFNG or IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. Through cell signaling, interferon gamma plays a role in regulating the immune response of its target cell. A key signaling pathway that is activated by type II IFN is the JAK-STAT signaling pathway. IFNG plays an important role in both innate and adaptive immunity. Type II IFN is primarily secreted by adaptive immune cells, more specifically CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The expression of type II IFN is upregulated and downregulated by cytokines. By activating signaling pathways in cells such as macrophages, B cells, and CD8+ cytotoxic T cells, it is able to promote inflammation, antiviral or antibacterial activity, and cell proliferation and differentiation. Type II IFN has played a role in the development of cancer immunotherapy treatments due to its ability to prevent tumor growth.
Standard curve
Example of Rat IFN-γ standard curve in Assay Diluent #1.
Standard curve
Example of Rat IFN-γ standard curve in Assay Diluent #1R1000.
Linearity
The concentrations of Rat IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Rat splenocyte cells stimulated with 5 µg/mL of Concanavalin A for 48 hours （1.25%）. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5999.01 pg/mL in stimulated Rat splenocyte cells.
Linearity
The sample is undiluted samples are as follows: Rat splenocyte cells culture for 48 hours （50%）. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 573.93 pg/mL in unstimulated Rat splenocyte cells.
To assess the linearity of the assay, in cell culture media with high concentrations of Rat IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, in serum and plasma with high concentrations of Rat IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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