PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:50 | Hu |
PDLIM7, also known as LMP1 or Enigma, is a protein encoded by the PDLIM7 gene and belongs to the family of PDZ-LIM proteins. It contains a PDZ domain and three LIM domains, which are involved in protein-protein interactions. The PDZ domain binds to actin filaments, while the LIM domains interact with protein kinases. PDLIM7 functions as an intracellular scaffold protein and acts as an adapter, localizing LIM-binding proteins to actin filaments in both skeletal muscle and nonmuscle tissues. It is involved in various biological processes, including the assembly of actin filament-associated complexes essential for signaling, regulation of mesenchymal stem cell osteogenesis, and promotion of cell proliferation. In thyroid cancer, PDLIM7 is overexpressed in a stage-dependent manner and interacts with pathways such as PI3K/AKT, MDM2, and BMP-1, suggesting its potential role in cancer progression.
WB result of PDLI7/LMP1 Recombinant Rabbit mAb
Primary antibody: PDLI7/LMP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: WI-38 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PDLI7/LMP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-PDLI7/LMP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HepG2 cells. Anti- PDLI7/LMP1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of PDLI7/LMP1 in human tissue.
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