PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu, Ms |
SLC25A51, also known as MCART1, is a mammalian mitochondrial NAD+ transporter that plays a crucial role in cellular metabolism and energy production. Located in the inner mitochondrial membrane, it facilitates the transport of NAD+ into mitochondria, which is essential for maintaining mitochondrial NAD+/NADH ratios and supporting mitochondrial respiration. SLC25A51 is involved in various cellular processes, including the regulation of the AKT/mTOR signaling pathway and proline synthesis, which are critical for cell proliferation. Additionally, its expression is regulated by fasting and circadian rhythms, and it is necessary for sustaining the activity of the mitochondrial deacetylase SIRT3. Recent studies have also shown that SLC25A51 is upregulated in various cancers and promotes tumor growth by maintaining mitochondrial acetylation homeostasis.
WB result of SLC25A51 Recombinant Rabbit mAb
Primary antibody: SLC25A51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: SW480 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: SW620 whole cell lysate 20 µg
Lane 5: LoVo whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SLC25A51 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of SLC25A51 in human tissue.
Expression of SLC25A51 in mouse & rat tissue.
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