PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
EGR1 protein, also known as Early Growth Response 1 or NGFI-A, is a mammalian transcription factor encoded by the EGR1 gene. It belongs to the EGR family of Cys2His2-type zinc finger proteins and acts as a nuclear protein regulating transcription. EGR1 plays a crucial role in various biological processes, including cell differentiation, proliferation, and apoptosis. It is involved in the regulation of multiple genes, some of which are tumor suppressors like TGFβ1, p53, and PTEN. However, its role in cancer is complex; while it is overexpressed in some cancers like colorectal and gastric cancer, leading to poor prognosis, it also acts as a tumor suppressor in others, such as rhabdomyosarcoma. Additionally, EGR1 is implicated in neuronal plasticity and cardiovascular diseases.
WB result of EGR1 Rabbit pAb
Primary antibody: EGR1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-α for 1 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 85 kDa
WB result of EGR1 Rabbit pAb
Primary antibody: EGR1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 85 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-EGR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of EGR1 in tumor tissue
Expression of EGR1 in human tissue
Expression of EGR1 in mouse & rat tissue.
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