PBS, 0.3% Proclin 300
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
SD Rat splenocytes were stained with SDT FITC Mouse IgG2a, κ Isotype Control Antibody (Right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (Left panel) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software