PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Ms |
PD-L1, also known as CD274 or B7-H1, is a type I transmembrane glycoprotein and a member of the B7 family. It was first identified in 1999. PD-L1 plays a crucial role in immune regulation by binding to PD-1 on T cells, inhibiting their activation and proliferation through multiple pathways, thus dampening tumor-reactive T cell responses. This protein is widely expressed in various cells, including antigen-presenting cells, activated T cells, B cells, and some non-hematopoietic cells. Its expression is often upregulated in tumor cells, contributing to immune evasion. Targeting PD-L1 has become a major strategy in cancer immunotherapy, with several antibodies developed to block its interaction with PD-1, enhancing anti-tumor immunity.
Flow cytometric analysis of Mouse CD274/B7-H1/PD-L1 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD274/B7-H1/PD-L1 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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