PBS, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu |
CD20, also known as B-lymphocyte antigen CD20, is a 33–37 kDa non-glycosylated protein primarily expressed on the surface of B cells from the pre-B cell stage through maturity, but it is absent on plasma cells. It is encoded by the MS4A1 gene and belongs to the membrane-spanning 4A gene family. CD20 is a transmembrane protein with four hydrophobic transmembrane domains, one intracellular domain, and two extracellular loops. Although its exact biological function remains unclear, it is believed to play a role in B cell activation and proliferation by facilitating calcium influx. CD20 is an important therapeutic target for B cell malignancies such as chronic lymphocytic leukemia and non-Hodgkin lymphoma, as well as for some autoimmune diseases.
Flow cytometric analysis of Human CD20 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE-Cy7 Mouse Anti-Human CD19 Antibody and either APC-Cy7 Mouse IgG2b, κ Isotype Control (Left panel) or SDT APC-Cy7 Mouse Anti-Human CD20 Antibody (Right panel) at 5 μl/test and treated with True-Stain Monocyte Blocker™ separately. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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