PBS, 0.3% Proclin 300
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
SD Rat splenocytes were stained with SDT Biotin Mouse IgG2a, κ Isotype Control Antibody (Right panel) at 1/20 dilution (1 μg) followed by Sav-iFluor 488. Cells without incubation with primary antibody and secondary antibody (Left panel) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.