PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| FCM | 1:20000 | Hu |
FcγRIa/CD64 is a high-affinity receptor for the Fc portion of IgG antibodies, playing a crucial role in immune responses. It is an integral membrane glycoprotein composed of a signal peptide, three extracellular immunoglobulin domains of the C2-type for antibody binding, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is primarily found on macrophages and monocytes but can be induced on polymorphonuclear leukocytes by cytokines like IFNγ and G-CSF. This receptor has the highest affinity for IgG1 among all Fcγ receptors, making it significant for monoclonal antibody binding and effector functions. It interacts with the common γ chain to trigger cellular activation upon binding to IgG. There are three distinct genes for CD64 in humans—FcγRIA (CD64A), FcγRIB (CD64B), and FcγRIC (CD64C)—located on chromosome 1, which produce six different mRNA transcripts through alternative splicing.
WB result of Fc γ RIa/CD64 Mouse mAb
Primary antibody: Fc γ RIa/CD64 Mouse mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: U937 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40, 55-70 kDa
This blot was developed with high sensitivity substrate
Flow cytometric analysis of human peripheral blood cells labelling Fc γ RIa/CD64 antibody at 1/20000 (0.01 μg) dilution/ (Right panel) compared with a Mouse IgG2b, κ Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with I-A/I-E - APC antibody separately.
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