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Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb (S-1529-102)

Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb (S-1529-102)

货号: S0B6019
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-GSK3α/β (Ser21/9)

  • 分子别名

    Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm, Nucleus

  • Accession

    P49840, P49841

  • 克隆号

    S-1529-102

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
背景介绍

  • Phospho-GSK3α/β (Ser21/9) refers to the phosphorylated forms of glycogen synthase kinase 3 alpha (GSK3α) at serine 21 and glycogen synthase kinase 3 beta (GSK3β) at serine 9. The phosphorylation at these sites is inhibitory, meaning it reduces the activity of these kinases. GSK3α/β are serine/threonine kinases that play crucial roles in various cellular processes, including cell fate determination, glucose homeostasis, and Wnt signaling. When phosphorylated at Ser21/9, the activity of GSK3α/β is suppressed, which can have significant impacts on these pathways. For example, in the Wnt signaling pathway, the inhibition of GSK3β by phosphorylation at Ser9 prevents the degradation of β-catenin, allowing it to accumulate and activate downstream gene expression. This phosphorylation is often regulated by upstream signals such as insulin or growth factors, which can induce the phosphorylation of GSK3α/β at these sites through various signaling cascades.

  • 免疫印迹

    • WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
      Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47, 51 kDa
      Observed MW: 45, 51 kDa

    • WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
      Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: Serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47, 51 kDa
      Observed MW: 45, 55 kDa

    • WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
      Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47, 51 kDa
      Observed MW: 47, 51 kDa

  • 斑点杂交

    • Dot blot result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
      Lane 1: GSK-3β (Ser9) phospho peptide
      Lane 2: GSK-3β (Ser9) unmodified peptide
      Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution