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Invivo Anti-Mouse CD40 Recombinant mAb (S-R456)

Invivo Anti-Mouse CD40 Recombinant mAb (S-R456)

货号: S0B7000
价格: 900
规格: 1mg
介绍: -
其他: -
产品规格
  • 宿主来源

    Rat
  • 抗原名称

    CD40
  • 分子别名

    Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; Tnfrsf5
  • 细胞定位

    Secreted, Cell membrane
  • Accession

    P27512
  • 克隆号

    S-R456
  • 抗体类型

    Rat mAb
  • 抗体同种型

    Rat IgG2a,k
  • 应用

    in vitro B cell stimulation/activation, in vivo CD40 activation, FCM
  • 反应种属 ?

    Ms
  • 阳性样本

    C57BL/6 mouse splenocytes
  • 纯化方式

    Protein G
  • 浓度

    5 mg/ml
  • 内毒素含量

    <2EU/mg
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions; 

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

稀释度
应用 稀释度 推荐种属
FCM 1:250 Ms
背景介绍
  • This antibody recognizes the same epitope as clone FGK45. CD40, also known as TNFRSF5, is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily, primarily expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. Its ligand, CD40L (CD154), is mainly found on activated T cells and is also expressed on platelets, endothelial cells, and smooth muscle cells. The interaction between CD40 and CD40L plays a crucial role in immune regulation, mediating a wide range of immune responses, including B cell proliferation, differentiation, and immunoglobulin class switching, as well as enhancing T cell activation and dendritic cell maturation. This interaction initiates intracellular signaling pathways involving TRAF proteins and leads to the activation of transcription factors such as NF-κB, which in turn regulate cytokine production and immune cell function. CD40 signaling is also implicated in metabolic processes, influencing glucose metabolism and lipid homeostasis, particularly in macrophages and endothelial cells. Dysregulation of the CD40-CD40L pathway is associated with various diseases, including immunodeficiency syndromes and autoimmune disorders.

  • 流式分析

    • Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD40 antibody at 1/250 (2 μg) dilution (Right) compared with a Rat IgG2a, κ isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.