PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu |
CD20, also known as B-lymphocyte antigen CD20, is a 33–37 kDa non-glycosylated protein primarily expressed on the surface of B cells from the pre-B cell stage through maturity, but it is absent on plasma cells. It is encoded by the MS4A1 gene and belongs to the membrane-spanning 4A gene family. CD20 is a transmembrane protein with four hydrophobic transmembrane domains, one intracellular domain, and two extracellular loops. Although its exact biological function remains unclear, it is believed to play a role in B cell activation and proliferation by facilitating calcium influx. CD20 is an important therapeutic target for B cell malignancies such as chronic lymphocytic leukemia and non-Hodgkin lymphoma, as well as for some autoimmune diseases.
Flow cytometric analysis of Human CD20 expression on human peripheral blood cells. Human peripheral blood cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 and either Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD20 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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