2 to 8 °C as supplied.
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type-II T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 can be produced by monocytes upon PD-1 triggering in these cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
Standard curve
Example of NIBSC (93/722) standard curve in Assay Diluent #12.
Standard curve
Example of human IL-10 standard curve in Assay Diluent #12.
Linearity
The concentrations of Human IL-10 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 2days (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4895 pg/mL in stimulated human PBMC supernatant.
Linearity
The sample is undiluted samples are as follows: human PBMC cells culture for 2days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 58.81pg/mL in unstimulated human PBMC supernatant.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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