PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Dynamin-related protein 1 (DRP1) is a cytoplasmic GTPase protein encoded by the DNM1L gene, playing a crucial role in mediating mitochondrial fission. Under normal conditions, DRP1 resides in the cytoplasm and is recruited to the mitochondrial outer membrane upon activation, where it forms spiral oligomers that constrict and divide the mitochondria. This process is regulated by various post-translational modifications, such as phosphorylation at serine 616, which enhances DRP1 activity and promotes mitochondrial fission. DRP1 is essential for maintaining mitochondrial dynamics, cell survival, and energy metabolism, and its dysregulation has been implicated in a range of diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases.
WB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 17 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 85 kDa
WB result of Phospho-DRP1 (Ser616) Recombinant Rabbit mAb
Primary antibody: Phospho-DRP1 (Ser616) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 85 kDa
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