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Phospho-IκBα (Ser32) Recombinant Rabbit mAb (S-1084-395)

Phospho-IκBα (Ser32) Recombinant Rabbit mAb (S-1084-395)

货号: S0B6000
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Phospho-IκBα (Ser32)
  • 分子别名

    NF-kappa-B inhibitor alpha; I-kappa-B-alpha (IkB-alpha; IkappaBalpha); Major histocompatibility complex enhancer-binding protein MAD3; IKBA; MAD3; NFKBI; NFKBIA
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Nucleus
  • Accession

    P25963
  • 克隆号

    S-1084-395
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    WB
  • 反应种属 ?

    Hu, Ms
  • 预测反应种属
    (反应种属缩写表)

    Pg
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu, Ms
背景介绍
  • Phospho-IκBα (Ser32) is a phosphorylated form of the IκBα protein, which plays a crucial role in the NF-κB signaling pathway. IκBα normally inhibits NF-κB by binding to it and preventing its nuclear translocation. However, when IκBα is phosphorylated at Ser32 (and often also at Ser36), it undergoes ubiquitination and subsequent degradation by the 26S proteasome, thereby releasing NF-κB to translocate to the nucleus and activate gene expression. This phosphorylation event is typically mediated by the IKK complex in response to various stimuli such as cytokines, growth factors, and stress signals. Antibodies specific to Phospho-IκBα (Ser32) are widely used in research to detect and quantify this phosphorylated protein, helping to elucidate the activation status of the NF-κB pathway in different cellular contexts.

  • 免疫印迹

    • WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 38 kDa

    • WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 38 kDa
      This blot was developed with high sensitivity substrate

  • 斑点杂交

    • Dot blot result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
      Lane 1: IκBα (Ser32) phospho peptide
      Lane 2: IκBα (Ser32) unmodified peptide
      Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution