2 to 8 °C as supplied.
Human RBP4, a lipocalin-family lipid carrier, transports retinol (vitamin A) from hepatic stores to peripheral tissues through its conserved β-barrel structure, circulating in plasma as a complex with transthyretin (TTR) to stabilize delivery. Structural integrity is maintained by disulfide bonds, while glycosylation (e.g., at Asn-95) modulates solubility and interaction dynamics. Monkey RBP4 shares 97% sequence identity with human RBP4, including identical retinol-binding residues (e.g., Thr-67, Gln-98). RBP4 facilitates retinol uptake via the STRA6 receptor, ensuring cellular vitamin A distribution critical for metabolic and immune functions. Elevated RBP4 levels are implicated in insulin resistance, adipose tissue inflammation, and type 2 diabetes pathogenesis by disrupting metabolic signaling pathways. Additionally, it regulates immune responses through retinol-dependent modulation of dendritic cell activity and T-cell function, linking vitamin A metabolism to inflammatory and autoimmune processes. This protein’s dual roles in nutrient transport and disease mechanisms underscore its importance in metabolic health and therapeutic research. Advantages of this OneStep ELISA Kit Design: Human & Monkey RBP4 OneStep ELISA Kit enables rapid (～60 min), sensitive (0.125 ng/mL) detection across human and Monkey samples, with cross-testing of horse and donkey samples, leveraging their structural conservation for translational research in metabolism, nutrition, and therapeutic development targeting insulin resistance or chronic diseases. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable RBP4 quantification in time-sensitive or resource-limited settings.
Standard curve
Example of Human and Monkey RBP4 standard curve in Assay Diluent #9.
Linearity
The concentrations of RBP4 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Human serum was 5×10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 59.17 ug/mL in human serum.
Linearity
The sample is undiluted samples are as follows: Human plasma was 5×10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 51.53 ug/mL in human plasma.
Linearity
The sample is undiluted samples are as follows: Monkey serum was 1×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 35.55 ug/mL in Monkey serum.
Cross-reactivity
The concentrations of RBP4 were measured and interpolated from the target standard curves and corrected for sample dilution.
Horse serum was 1×10^4 and 2×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7272.28 ng/mL in horse serum.
Donkey serum was 1×10^4 and 2×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11058.44 ng/mL in donkey serum.
Leading Competitor comparison
您现在的位置：