PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
The SSEA-4 (Stage-Specific Embryonic Antigen-4) is a significant glycolipid antigen belonging to the Globo series glycosphingolipids (such as Gb5 and SSEA-3). Its structural characteristic is Neu5Gcα2-3Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-Cer. It is highly expressed during early embryonic development (e.g., in morula and blastocyst stages) and in pluripotent stem cells (such as human embryonic stem cells, hESCs, and induced pluripotent stem cells, iPSCs), making it a widely used surface marker for pluripotency. Additionally, SSEA-4 is aberrantly expressed in various cancers (e.g., breast cancer, lung cancer, gliomas) and is associated with tumor progression, metastasis, and immune evasion, potentially through the regulation of cell adhesion or signaling pathways (e.g., EGFR/ERK). Notably, since humans lack the enzyme to synthesize its sialic acid component (Neu5Gc), SSEA-4 expression may rely on exogenous uptake or non-canonical biosynthetic pathways. This unique feature also presents a potential therapeutic target for cancer treatment.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NTERA-2 (Human malignant multiple teratoma cells) labelling SSEA4 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) Isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: Raji
ICC shows positive staining in NTERA-2 cells. Anti-SSEA4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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