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TOMM40 Rabbit Polyclonal Antibody

TOMM40 Rabbit Polyclonal Antibody

货号: S0B1493
价格: 400
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    TOMM40
  • 分子别名

    Mitochondrial import receptor subunit TOM40 homolog; Protein Haymaker; Translocase of outer membrane 40 kDa subunit homolog; p38.5; C19orf1; PEREC1; TOM40
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Mitochondrion
  • Accession

    O96008
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HeLa, 293T, MCF7, BxPC-3, mouse heart, rat heart
  • 预测反应种属
    (反应种属缩写表)

    Bv
  • 纯化方式

    Immunogen Affinity
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
背景介绍
  • TOMM40 (Translocase of Outer Mitochondrial Membrane 40) is a protein encoded by the TOMM40 gene. It is a key component of the outer mitochondrial membrane translocase complex, which is essential for the import of precursor proteins into mitochondria. This protein is embedded in the outer mitochondrial membrane and plays a crucial role in mitochondrial function and biogenesis. Research has shown that TOMM40 is involved in various cellular processes, including lipid metabolism and energy production. Additionally, it has been implicated in several diseases, such as cardiovascular system disease and Alzheimer's disease.

  • 免疫印迹

    • WB result of TOMM40 Rabbit pAb
      Primary antibody: TOMM40 Rabbit pAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: BxPC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 38 kDa

    • WB result of TOMM40 Rabbit pAb
      Primary antibody: TOMM40 Rabbit pAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 38 kDa

    • WB result of TOMM40 Rabbit pAb
      Primary antibody: TOMM40 Rabbit pAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 38 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-TOMM40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TOMM40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TOMM40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-TOMM40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-TOMM40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of TOMM40 in tumor tissue.

    • Expression of TOMM40 in human tissue.

    • Expression of TOMM40 in mouse & rat tissue.